NCBI practice

Topic
1. mucopolysaccharidosis(nucleotide) 
-> add limiter "human[organism]"
-> Get the gene record through "mRNA" links on the right
-> select top1 mRNA information: Genebank. FASTA.Graphics
-> Get the OMIM record

2.fucosyltransferase 8(nucleotide)
->add limiter fucosyltransferase 8 OR FUT8 AND human[organism]
->Homo sapiens monocyte to macrophage differentiation-associated 2 (MMD2),
 transcript variant 1, mRNA

3.GALNT6(nucletide)  breast cancer-related
->add limiter "human[org]"
->get the RefSeq information
->FASTA & Graphics

4.CDG(nucleotide)   Congenital Disorder of Glycosylation
->add limiter "human[org]"
->Homo sapiens mannose phosphate isomerase (MPI), mRNA
->Get the OMIM informaion

5.OGT(nuclotide)  O-linked GlcNAc
                                                               

Entrez Gene practice

      Find genes by ....                                            Search text
           free  text                                                       galectin1
partial name and multiple species    
                                     alive[prop] AND lectin[title] AND ("Danio rerio "[orgn] OR "Mus musculus"[orgn])
   chromosome and symbol                           22[chr]) AND GBP*[sym]
associated sequence accession number                M57678[accn]
      gene name (symbol)                                      LGALS1[sym]
     publication (PubMed ID)                       1988031, 12271131[PMID]
     chromosome and species                         22[chr] AND human[orgn]

TOPIC - Glycan analysis (year & author)

TOPIC - Glycan analysis (total:3397) 分析取用前2500筆資料                            Publication Year Record Count % of 2500 2001 9 0.36% 2002 141 5.64% 2003 165 6.60% 2004 190 7.60% 2005 204 8.16% 2006 286 11.44% 2007 283 11.32% 2008 388 15.52% 2009 507 20.28% 2010 327 13.08% Author Record Count % of 2500 RUDD, PM 56 2.24% DELL, A 55 2.20% DWEK, RA 52 2.08% HARVEY, DJ 46 1.84% NARIMATSU, H 36 1.44% MORRIS, HR 31 1.24% NISHIMURA, SI 31 1.24% CUMMINGS, RD 30 1.20% DEELDER, AM 29 1.16% NAKAGAWA, H 29 1.16% (只取前面比較多的部分)

我眼中的分子生物學

分子生物學即是由central dogma為中心所衍生出來的生物相關科學,包括gemoics,proteomics以致到後來的modification均有相關,整體以基因體為主要架構來探討所有有關係的生物與生命現象